A1R Confocal – A1R Upright Multi-photon – Spinning disk systems

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A1R Confocal – A1R Upright Multi-photon – Spinning disk systems

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nic-kings-overview

On Github drmatthews / nic-kings-overview

Professor Maddy Parsons - NIC Director

Isma Ali - NIC Deputy Manager

Dr Dan Matthews - NIC Manager

3rd Floor Hodgkin Building - Guy's Campus

NIC@King's

  • Opened April 2012, over 200 trained users
  • KCL funded initial refurbishment of space and purchased 2 systems up-front. KCL cover annual maintenance contract for all systems. Nikon loan remaining systems and provide flexible upgrades/swap-outs
  • Managed by Dan Matthews and deputy manager Isma Ali (KCL employees). Maddy Parsons is founder and Academic Director
  • Overseen by steering committee made up of academic members of staff
  • Users charged for all systems - cost determined by system maintenance contract

Biomedical research at King's

  • Cancer - cell biology (cytoskeleton, adhesion, migration, invasion), stem cell biophysics, in vivo models
  • Neurobiology - in vivo developmental models, intracellular traffic, synapse assembly
  • Cardiovascular – regulation of cardiac myocytes, heart development in vivo, drug delivery
  • Asthma and allergy – inflammatory cell migration, apoptosis,drug screens
  • Immunology – immune cell traffic (in vitro, in vivo), virus assembly and infection

Workshops/Training/Demos

  • Masters and PhD workshops: combined lectures and hands-on practical training
  • External (non-KCL) collaborators and users (industry and academia)
  • Showcase site for Nikon for potential customers
  • Training site for Nikon staff
  • Beta-testing for new Nikon products

Overview of systems

Currently 12 systems

  • 2x inverted confocals
  • 1x upright confocal
  • 1x upright multiphoton
  • 2x live cell widefield fluorescence time-lapse
  • 1x N-STORM
  • 1x N-SIM
  • 2x spinning disk confocal
  • 2x Biostations

A1R Confocal

  • Configured for speed and sensitivity
  • Can be used for live or fixed samples
  • Resonant scanner and piezo z-drive
  • GaAsp high sensitivity detectors
  • NIC@King’s Image Competition winner 2013 – Kif Liathakula
  • Hair folicles in mouse epidermis

A1R Confocal

A1 Upright Confocal

  • Owned by Prof Fiona Watt
  • Imaging of stem cells and mouse skin/hair follicles
  • Focus on low-magnification/macro-imaging
  • Shared usage with other centre users (70/30)

A1R Upright Multi-photon

  • Good for deep tissue imaging (in vivo)
  • Has resonant scanner and piezo z-drive
  • Can also be used as a standard upright confocal

Spinning disk systems

  • Variety of projects – lots of live cell imaging
  • Inverted has NEO sCMOS, upright has iXON Ultra EMCCD

Biostation

  • Microscope in-a-box for very long term imaging
  • Variety of projects: T-cell interactions/wound healing
  • Popular because of low maintenance and ease of use

Live cell imaging

  • 1x system equipped with C2 confocal scan head
  • 1x system has sCMOS and RGB camera for H&E samples

Structured Illumination - NSIM

  • 2-fold improvement in spatial resolutuon compared with confocal
  • Also configured for TIRF-SIM
  • Stage-top incubator for live cell imaging

NSTORM

  • 20-40nm resolution in XY
  • Capable of nSTORM, dSTORM and PALM
Professor Maddy Parsons - NIC Director Isma Ali - NIC Deputy Manager Dr Dan Matthews - NIC Manager